Laboratory for Molecular Diagnostics
Center for Nephrology and Metabolic Disorders
Moldiag Diseases Genes Support Contact

Sample requirements

Sample:

Generally, it is possible to get genomic DNA from almost all nucleus containing cells in the body. Frequently white blood cell from the peripheral blood stream are used for preparation of genomic DNA. We prefer blood samples with EDTA as anticoagulant. 1 to 5 Millilitres are sufficient for a wide range of test.

For a simple single test sufficient DNA can be processed from a buccal swap.

To ease international transport, it is sometimes recommended to mail extracted DNA. If prepared DNA is mailed, please indicate the method of preparation because this can influence some of the following test procedures. We usually receive 20-40µg of DNA. And again, family screening or prenatal diagnosis for a known mutation needs much less DNA. The exact amount of DNA required for a specific test is listed on our price offer sheet that is mailed on request.

All samples mailed internationally must be not infectious. Please declare the method by which this was excluded. In most cases it will be sufficient to do this clinically only. In general, this is enough. But if the patient was tested for some reasons, please indicate.

Please indicate this in our forms and carefully read the shipment instructions to learn how to indicate it at the accompanying papers.

It is much more difficult to prepare RNA. This type of nucleic acid is not stable and easily degraded by ubiquitary RNAses even under sterile conditions. If your are planning to send a sample in for an RNA test, please ask for sampling and transport material.

Genomic DNA:

Generally, it is possible to get genomic DNA from almost all nucleus containing cells in the body. Frequently white blood cell from the peripheral blood stream are used for preparation of genomic DNA. We prefer blood samples with EDTA as anticoagulant. 1 to 5 Millilitres are sufficient for a wide range of test.

For a simple single test sufficient DNA can be processed from a buccal swap.

To ease international transport, it is sometimes recommended to mail extracted DNA. If prepared DNA is mailed, please indicate the method of preparation because this can influence some of the following test procedures. We usually receive 20-40µg of DNA. And again, family screening or prenatal diagnosis for a known mutation needs much less DNA. The exact amount of DNA requiered for a specific test is listed on our price offer sheet that is mailed on request.

All samples mailed internationally must be not infectious. Please declare the method by which this was excluded. In most cases it will be sufficient to do this clinically only. In general, this is enough. But if the patient was tested for some reasons, please indicate.

Please indicate this in our forms and carefully read the shipment instructions to learn how to indicate it at the accompanying papers.

Generally, it is possible to get genomic DNA from almost all nucleus containing cells in the body. Frequently white blood cell from the peripheral blood stream are used for preparation of genomic DNA. We prefer blood samples with EDTA as anticoagulant. 1 to 5 Millilitres are sufficient for a wide range of test.

For a simple single test sufficient DNA can be processed from a buccal swap.

To ease international transport, it is sometimes recommended to mail extracted DNA. If prepared DNA is mailed, please indicate the method of preparation because this can influence some of the following test procedures. We usually receive 20-40µg of DNA. And again, family screening or prenatal diagnosis for a known mutation needs much less DNA. The exact amount of DNA requiered for a specific test is listed on our price offer sheet that is mailed on request.

All samples mailed internationally must be not infectious. Please declare the method by which this was excluded. In most cases it will be sufficient to do this clinically only. In general, this is enough. But if the patient was tested for some reasons, please indicate.

Please indicate this in our forms and carefully read the shipment instructions to learn how to indicate it at the accompanying papers.

It is much more difficult to prepare RNA. This type of nucleic acid is not stable and easily degraded by ubiquitary RNAses even under sterile conditions. If your are planning to send a sample in for an RNA test, please ask for sampling and transport material.

The samples should be wrapped in firm and stable vessels to prevent disruption during international transport.

Please assure that the sample is stored under sterile conditions to avoid deteriorations by bacteria or fungi. No other conditions are required. The DNA samples can be shipped at room temperature it must not be chilled. Avoid freezing the sample because water crystals use to break DNA strands.

Update: Aug. 14, 2020
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Albert-Schweitzer-Ring 32, D-02943 Weißwasser, Germany, Tel.: +49-3576-287922, Fax: +49-3576-287944
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